Composite

Part:BBa_K4674013:Design

Designed by: Chun Ying Yu   Group: iGEM23_CCU-Taiwan   (2023-10-08)


The PBSII-Zif268 expression cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 424
    Illegal BamHI site found at 711
    Illegal XhoI site found at 706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nope


Source

The expression cassette is composed of T7 promoter, Lac operon, RBS, PBSII, Zif268, and T7 terminator.

References